Top Page > PROUSION
Effects of prousion on rabbit models with high plasma lipid
PAN Jia-Hu, HASHIMOTO Masakazu1, CHANG Jian-Jie, NIE Jin, JIN Jian
Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 200032, China; 1Japan Health Promotion Association, 7-17-801 Nihonbashi Yokoyama-cho, Chuou-ku, Tokyo 103-0003, Japan.
AIM:
To check the toxicity of prousion-B and detect its effects on the rabbit models with high plasma lipid.METHODS:
- The acute toxicity of P-B suspended with 0.2 % CMC was performed on the mice of Kunming species.
- The rabbit models with high plasma lipid were used to determine the effects of P-B in vivo, ie, the rabbits were divided into 6 groups: blank control, model, positive control (nicotinic acid), P-B in different dosage (0.1, 0.2, and 0.4 g/kg). The rabbits were given ig with the above drugs once a day at the same time with high lipid food for 6 weeks.
- The blood lipid levels were checked in 2 weeks and the blood samples were taken for the examination of some enzyme activities reflecting the heart and liver functions.
RESULTS:
- The dosage of P-B in the largest suspension concentration (3 g/kg) did not induce death of the mice, showing that the toxicity of P-B was low.
- Although there was no significant difference between the rabbit groups given with P-B and the control in statistics, P-B showed a tendency to decrease blood total cholesterol (TC), low-density lipoprotein (LDL), and apolipoprotein B (apB) levels in the rabbits.
- P-B obviously inhibited the increase of total creatine kinase (TCK) activity and glutamic-pyruvic transaminase (GPT) and glutamic-oxaloacetic transaminase (GOT) levels induced by high plasma lipids in the rabbit models, reflecting that P-B had certain protective functions on the rabbit model with high plasma lipid.
- P-B showed no obvious effects on kidney function, blood
electrolyte levels, and erythrocyte index in rabbit models.
The effect for the active level of SOD and
GSH-Px and MDA in the high lipid blood plasma rabbit.
（X ± S, n =6）
| Group | SOD（U/ml） | GSH-Px （μmol.L－1.min－1） |
MDA （μ mol/L） |
|---|---|---|---|
| Control |
4,574.50 ± 881.90
|
35.55±5.00
|
6.36±0.61
|
| Model |
4,855.50±2,880.59
|
45.56±10.76
|
5.73±0.31
|
| Vpp |
6,661.50±2,105.06
|
24.52±0.8374
|
5.38±0.05
|
| Ｐ (0.1g/Kg) |
2,809.67 ± 932.42
|
37.10±8.25
|
5.13±0.50
|
| Ｐ (0.2g/Kg) |
7,842.50 ± 217.08
|
41.66±4.52
|
5.31±0.93
|
| Ｐ (0.4g/Kg) |
6,340.75 ± 2,088.17
|
6,340.75 ± 2,088.17
|
5.07±0.61
|
Vpp：The group which uses the nicotinic
acid vitamin
SOD：Superoxide dismutase
GSH-Px：Glutathione reductase
MDA：Malondialdehyde
SOD：Superoxide dismutase
GSH-Px：Glutathione reductase
MDA：Malondialdehyde
CONCLUSION:
PROUSION-B had certain protective functions on the rabbit model with high plasma lipid.
Effects of prousion on scavenging free radicals
